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Home > A sensitive immunoassay for flunitrazepam and metabolites.

Walshe, K and Barrett, AM and Kavanagh, PV and McNamara, SM and Moran, C and Shattock, AG (2000) A sensitive immunoassay for flunitrazepam and metabolites. Journal of Analytical Toxicology , 24 , (4) , pp. 296-299.

The objective of this study was to develop an immunoassay which would be capable of detecting flunitrazepam and/or cross-reacting metabolites in urine and comparing the results with those obtained by gas chromatoghraphy /mass spectroscopy (GC/MS).

Doses of RohypnolTM varying between 0.5 mg and 4 mg were given to volunteers and urine collected for up to two weeks post ingestion. These samples were analysed by an ELISA which was developed using an antibody raised to flunitrazepam and a drug-enzyme conjugate prepared by attaching 7-ammoflunitrazepam to horseradish peroxidase. Significant levels of flunitrazepam and/or cross-reacting metabolites were detected in urine for up to one week after ingestion. The immunoassay is selective with only diazepam cross-reacting at a level of 1000 ug/L.


Item Type
Article
Date
May 2000
Call No
CQ, BH
Page Range
pp. 296-299
Publisher
Preston Publications
Volume
24
Number
4
Keywords
flunitrazepam, gas chromatography, immunoassay, mass spectrometry
EndNote
Accession Number
HRB 1459 (Available)

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