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Home > E-cigarettes compromise the gut barrier and trigger inflammation.

Sharma, Aditi and Lee, Jasper and Fonseca, Ayden G and Moshensky, Alex and Kothari, Taha and Sayed, Ibrahim M and Ibeawuchi, Stella-Rita and Pranadinata, Rama F and Ear, Jason and Sahoo, Debashis and Crotty-Alexander, Laura E and Ghosh, Pradipta and Das, Soumita (2021) E-cigarettes compromise the gut barrier and trigger inflammation. iScience , 102035 . (In Press)


E-cigarette usage continues to rise, yet the safety of e-cigarette aerosols is questioned. Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics and murine and human gut-derived organoids in co-culture models, we assessed the effects of e-cigarette use on the gut barrier. Histologic and transcriptome analyses revealed that chronic, but not acute, nicotine-free e-cigarette use increased inflammation and reduced expression of tight junction markers (TJ). Exposure of murine and human enteroid-derived monolayers (EDMs) to nicotine-free e-cigarette aerosols alone or in co-culture with bacteria, also cause barrier-disruption, downregulation of TJ protein, enhanced inflammation in response to infection. These data highlight the harmful effect of “non-nicotine” component of e-cigarette on gut barrier. Considering the importance of an intact gut barrier for host fitness, and the impact of gut mucosal inflammation on a multitude of chronic diseases, these findings are broadly relevant to both medicine and public health.

Item Type
Publication Type
International, Open Access, Article
Drug Type
Tobacco / Nicotine
Intervention Type
Harm reduction
January 2021
Identification #

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