The Strategic Plan for an Integrated Public Health Laboratory System in the Americas 2025–2030 focuses on strengthening the capacities of laboratory systems in the Region, in line with the provisions of the International Health Regulations (2005), the Disease Elimination Initiative, the One Health approach, and the Sustainable Development Goals. This plan promotes the work of laboratory networks operating at different levels of care, each equipped with adequate infrastructure, trained human resources, and efficient management of supplies and materials. It also promotes industry leadership in quality, biological risk management, research, and communication. Public health laboratories are a key player at the human-animal-environment interface, especially during health crises such as outbreaks, epidemics, pandemics, and natural disasters. They play a fundamental role in mitigating damage to the population and accelerating the elimination of communicable diseases, particularly in the most vulnerable groups. By generating scientific results and evidence, these laboratories provide critical information that allows authorities to make decisions and implement effective measures to protect human and animal health, ensure food safety, and conserve environmental biodiversity. Resilience in the face of health emergencies requires a laboratory system with technical capacity, sustainable funding, and strong governance. This plan also fosters innovation and intersectoral partnerships, and seeks to optimize access to and use of advanced technologies to address public health emergencies of international importance in a timely and efficient manner. By promoting integrated management and operational continuity in crisis situations, public health laboratories are essential for early recovery and contribute to the elimination of communicable diseases in the Region of the Americas.