Census 2022 reveals that the growth in the Irish population is accompanied by significant diversity in national origin, ethnicity and religious affiliation. It was the first time that the Irish population exceeded 5 million in a census since 1851. Migration was a significant factor in population growth. This population growth builds on the diversity of religious communities and ethnic groups that were already present in Ireland. Increasing diversity is evident among both those who use the HSE, and the staff who provide these services. The First Intercultural Guide was originally published in 2009. This fully revised version of the Guide was developed in response to an expressed need for an intercultural guide and resource by healthcare staff across a range of cultural backgrounds. Staff have sought relevant information to help build knowledge, skills and awareness in order to deliver care to people from backgrounds other than their own. To this end, the Guide profiles the religious and cultural needs of twenty-five diverse groups who are being cared for in healthcare settings. These groups comprise twenty-one religious groups, 3 ethnic/cultural groups and those without religious belief. As Irish society continues to become increasingly diverse, new measures have been developed to enable the health system to respond appropriately to the needs of service users from all groups. This Guide was revised under the HSE Second National Intercultural Health Strategy (2018-2023) as part of a framework of initiatives designed to build and enhance capacity to deliver culturally competent care in Irish health settings.